Regulation of Gene Expression
For Student Groups
How are genes regulated at different levels? This course focuses on the different and interconnected processes of gene regulation.
Book Regulation of Gene Expression
- Age
- 16 to 20 years
- Duration
- 1 day
- Maximum number of participants
- 20
Experiments
- Protein isolation from different pig organs
- Analysis of the different protein expression patterns using SDS gel electrophoresis and coomassie staining
Gene expression plays a crucial role in determining cellular RNA and protein production, which influences the identity and function of cells, tissues and organs. Our course introduces the various mechanisms of gene regulation that occur at different levels: Before transcription, during transcription, after transcription, during translation and after translation. Topics covered include histone modifications, DNA methylation, transcription factors, RNA interference, splicing and microRNAs. In addition, specific processes such as hemoglobin switching and X-chromosome inactivation are explained. The practical focus of the course is on investigating the differences between various organs at the subcellular level. Analysis of protein levels in different organs reveals differences in gene regulation.
Keywords
Protein biosynthesis; epigenetics; RNA interference; genetic imprinting; gel electrophoresis