From Gene to Protein in Prokaryotic Cells
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  • Photo Department Molecular Biologie, From Gene to Protein in Prokaryotic Cells

Standard molecular methods are applied in this course to analyze the existence and expression of the GFP (green fluorescent protein) in bacteria.

Book From Gene to Protein in Prokaryotic cells

Age
16 to 20 years
Duration
2 days
Maximum number of participants
20

Experiments

  • Isolation of plasmid DNA
  • Restriction of DNA and analysis by agarose gel electrophoresis
  • Transformation and selection of bacterial cells
  • Polymerase chain reaction (PCR)
  • Denaturation of proteins

The reporter gene encoding the green fluorescent protein (GFP) originates from the jellyfish Aequorea victoria and is a useful tool in molecular biology research. In this course, modern molecular methods, are applied to study the existence and expression of GFP in Escherichia coli cells on DNA and protein level. After DNA isolation from bacteria, restriction analysis confirms its identity. Bacterial cells will be genetically transformed and selected. Successful transformation will be confirmed by PCR and gel electrophoresis. Furthermore, the effect of denaturating agents, such as high temperatures and different chemicals, on the fluorescence of GFP will be analyzed.