From Gene to Protein in Prokaryotic Cells
For Student Groups
Standard molecular methods are applied in this course to analyze the existence and expression of the GFP (green fluorescent protein) in bacteria.
- 16 to 20 years
- 2 days
- Maximum number of participants
- Isolation of plasmid DNA
- Restriction of DNA and analysis by agarose gel electrophoresis
- Transformation and selection of bacterial cells
- Polymerase chain reaction (PCR)
- Isolation of proteins and analysis by SDS-PAGE (polyacrylamide gel electrophoresis)
The reporter gene encoding the green fluorescent protein (GFP) originates from the jellyfish Aequorea victoria and is a useful tool in molecular biology research. In this course, modern molecular methods, such as genetic transformation of bacteria, DNA isolation, restriction analysis, PCR and SDS-PAGE are applied to study the existence and expression of GFP in Escherichia coli cells on DNA and protein level.