From Gene to Protein in Eukaryotic Cells
For Student Groups
Modern molecular methods are used in this course to identify and trace different fluorescent proteins in cultured eukaryotic cells.
- 16 to 20 years
- 2 days
- Maximum number of participants
- Isolation of plasmid DNA
- Transformation of DNA into eukaryotic cells
- Fluorescence microscopy
- Restriction analysis of DNA
- Polymerase chain reaction (PCR)
- Agarose gel electrophoresis
Eukaryotic cell culture offers a good model system to perform basic research in different fields, especially in medicine. Fluorescence proteins, such as GFP (green fluorescent protein) and RFP (red fluorescent protein) are useful tools to in vivo trace reactions and pathways. In this course, the plasmid encoding either the GFP or the RFP are isolated from bacterial cells and transformed into cultures eukaryotic cells. Molecular methods like restriction analysis and PCR will be used to find out which type of reporter gene was isolated; the results will later be confirmed by fluorescence microscopy.