Molecular analysis of the sickle cell disease

Genetic disorders are caused by mutations of DNA. Mutations can affect regions of various sizes, ranging from a single nucleotide to the whole chromosome. In case of the sickle cell disease a point mutation in the ß-globin gene changes the structure of the hemoglobin molecule, resulting in its disfunction.

In this course the sickle cell disease will be analyzed using molecular techniques. The unaffected and the mutated variant of the ß-globin gene will be examined by restriction analysis. The resulting DNA fragments are different in length (restriction fragment length polymorphism: RFLP) and can be visualized by agarose gel electrophoresis.

The following molecular techniques are carried out during the course:

Isolation of plasmid DNA
Restriction analysis
Agarose gel electrophoresis

 

Duration: 1 days
Number of participants: max. 20