From Gene to Protein

The expression of the reporter gene GFP, which encodes the green fluorescent protein, will be analyzed in Escherichia coli bacterial cells.

Structure and function of DNA and proteins, plasmid DNA, regulation of gene expression, operon regulation and genetic transformation represent the topics treated in this course.

The following molecular techniques are carried out during the course:

2 days course:

  • Isolation of plasmid DNA
  • DNA concentration
  • Transformation of plasmid DNA into E. coli bacteria
  • Polymerase chain reaction (PCR)
  • Restriction of Plasmid DNA
  • Agarose gel electrophoresis

 Additionally in the 21/2 days:

  • Protein analysis per SDS-PAGE

 

Duration: 2- 2 1/2 days

Participants: max. 20

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