Fluorescence microscopy of cellular structures

One of the most important light microscopic methods for the characterization of cellular structures (e. g. nucleus, lysosomes, cytoskeleton) is the immunofluorescence staining, which may also be used for the visualization of dynamic processes. The basis of this staining method is the high affinity between antigen and antibody. There are two different staining methods: direct and indirect immunoflourescence staining. In direct immunostaining the fluophore (dye molecule) is directly coupled to the antibody. In indirect immunostaining sort of a sandwich method is used. In a first step an antibody binds to the cellular target in the following second step this antigen-antibody complex is detected by a second antibody which is linked to the fluophore. The indirect immunostaining allows a strong amplification of the staining.
During the course both techniques are used to stain nuclei, several structures of the cytoskeleton and endosomes of cultured cells (fibroblasts).

Duration:
1 day
Number of participants: max. 20